Recombinant human PRMT5
Catalog # : EPX-012-RBV
Source : Human
Expressed in : SF9 cells
Quantity : 10µg of
enzymatically active recombinant PRMT5 at 0.5µg /µl
Arginine methyltransferase that can both catalyze the
omega-N monomethylarginine (MMA) and symmetrical dimethylarginine
(sDMA), with a preference for the formation of MMA (1). Methylates
histone H2A and H4 'Arg-3' during germ cell development. Methylates
histone H3 'Arg-8', which may repress transcription (2).
Recombinant human N-terminal Flag tagged PRMT5 was
produced in SF9
insect cells, purified using FPLC and formulated in a storage buffer
containing 20mM Tris-Cl pH 7.6, 1mM EDTA, 0.15 M NaCl, 10% glycerol,
0.5mM PMSF and 1mM DTT. Protein concentration was determined by
spectrometry. >95% purity by SDS-PAGE.
Each lot has been evaluated by 10% Tris Glycine SDS-PAGE.
Recombinant human histone H3-H4 tetramers (2 μg) were
a 25-μl reaction containing 50 mM Tris HCl, pH 8.0, 10% glycerol, 0.1
mM EDTA, 1 mM DTT, 3 nM at 5 nCi/μl of [3H] acetyl-CoA (Amersham
Pharmacia), and 0.2 pmol of purified recombinant FLAG-PRMT5. The
mixture was incubated at 30°C for 1 h.
For research use only. Products guaranteed stable for 2 years from date of receipt when stored properly.
>98% purity by SDS PAGE.
PRMT5 (Mw: 72,202 Da.)
1. Antonysamy S., Bonday
Z., Campbell R.M., Doyle
B., Druzina Z., Gheyi T., Han B., Jungheim L.N., Qian Y., Rauch C.,
Russell M., Sauder J.M., Wasserman S.R., Weichert K., Willard F.S.,
Zhang A., Emtage S.Proc. Natl. Acad. Sci. U.S.A. 109:17960-17965(2012)