Recombinant Histone H3.3
Catalog # : EPX-09-RH
Source : Human
Expressed in : E. Coli
Quantity : 100ug of recombinant histone H3.3 at 1ug /ul
The histone variant H3.3 replaces conventional H3.1 in a wide range of nucleosomes in active genes and constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis (1, 2).
Recombinant human histone H3.3 was produced in E. Coli, purified using FPLC and formulated in a storage buffer containing 20mM sodium phosphate pH 7.0, 1mM EDTA, 0.3 M NaCl, 0.5mM PMSF and 1mM DTT. Protein concentration was determined by spectrometry.
Each lot has been evaluated by ESI-TOF analysis and 12% SDS-PAGE (NuPAGE MOPS SDS, Invitrogen).
For research use only.
Products guaranteed stable for 2 years from date of receipt when stored properly.
>98% purity by SDS PAGE.
Human histone H3.3 (Theoretical Mw: 15196.72)
ARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGT VALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIG ALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA
1. Drané et al., (2010). Genes Dev. Jun 15;24(12):1253-65.
2. Ahmad and Henikoff. (2002). Mol Cell. Jun;9(6):1191-200.