Recombinant Histone H3.1-H4 tetramers

 

Catalog # : EPX-02-RH

 

Source : Human

 

Expressed in : E. Coli

 

Quantity : 50ug of recombinant H3.1-H4 tetramers at 1ug /ul

 

 

 

Background:

 

Histone H3-H4 tetramers are core components of the nucleosome (1). The nucleosome is basic unit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histones H2A, H2B, H3 and H4 (2).

 

 

Protein details:

 

Recombinant human histone H3-H4 dimers were produced in E. Coli as fusion proteins with an N-terminal 6xHis or FLAG epitopes, purified using FPLC and formulated in a storage buffer containing 20mM Tris-Cl pH 7.9, 1mM EDTA, 1M NaCl, 0.5mM PMSF and 1mM DTT. Protein concentration was determined by spectrometry.

 

 

Quality control:

 

Each lot has been evaluated by ESI-TOF analysis and 12% SDS-PAGE (NuPAGE MOPS SDS, Invitrogen).

 

center  
SDS-PAGE gel of recombinant Histone H3.1-H4 tertramers   ESi-TOF analysis of recombinant human N-terminal 6xHis-H3.1 and FLAG-H4 tetramers.

 

Application Notes:

 

For research use only.

 

 

Storage:

 

-80C

 

 

Guarantee:

 

Products guaranteed stable for 2 years from date of receipt when stored properly.

 

 

Purity:

 

>98% purity by SDS PAGE.

 

 

Protein sequences:

6xHis-H3.1 (Theoretical Mw: 16515.19

MGSHHHHHHGSARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPH

RYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAV

MALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

 

Flag-H4 (Theoretical Mw: 12717.75)

MIDYKDDDDKLESGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLA

RRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVY

ALKRQGRTLYGFGG

 

References:

1. Van Holde, K. E. (1989) Chromatin, 1-497.

2. Luger et al., (1997) Nature, 389(6648):251-60.

 

 

 


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