Recombinant Histone H2A-H2B dimers

 

Catalog # : EPX-01-RH

 

Source : Human

 

Expressed in : E. Coli

 

Quantity : 50ug of recombinant H2A-H2B dimers at 1ug /ul

 

 

 

Background:

 

Histone H2A-H2B dimers are core components of the nucleosome (1). The nucleosome is basic unit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histones H2A, H2B, H3 and H4 (2).

 

 

Protein details:

 

Recombinant human histone H2A-H2B dimers were produced in E. Coli as fusion proteins with an N-terminal FLAG or 6xHis epitopes, purified using FPLC and formulated in a storage buffer containing 20mM Tris-Cl pH 7.9, 1mM EDTA, 1M NaCl, 0.5mM PMSF and 1mM DTT. Protein concentration was determined by spectrometry.

 

 

Quality control:

 

Each lot has been evaluated by ESI-TOF analysis and 17% SDS-PAGE mini-gel (Tris-Glycine SDS, Acrylamide-bis (29:1)).

 

center  

SDS-PAGE gel of recombinant Histone H2A-H2B dimers.

  ESi-TOF analysis of recombinant human N-terminal FLAG-H2A and 6xHis-H2B dimers.

 

Application Notes:

 

For research use only.

 

 

Storage:

 

-80C

 

 

Guarantee:

 

Products guaranteed stable for 2 years from date of receipt when stored properly.

 

 

Purity:

 

>98% purity by SDS PAGE.

 

 

Protein sequences:

Flag-H2A (Theoretical Mw: 15328.72)

MDYKDDDDKLESGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNY

AERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRN

DEELNKLLGKVTIAQGGVLPNIQAVLLPKKTESHHKAKGK

 

6xHis-H2B (Theoretical Mw: 14887.10)

MGSHHHHHHGSPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSI

YVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTI

TSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSA

 

References:

1. Van Holde, K. E. (1989) Chromatin, 1-497.

2. Luger et al., (1997) Nature, 389(6648):251-60.

 

 

 


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