Recombinant Histone H2A.X

 

Catalog # :EPX-17-RH

 

Source : Human

 

Expressed in : E. Coli

 

Quantity : 100ug of recombinant histone H2A.X at 1ug /ul

 

 

 

Background:

 

The histone variant H2A.X is an evolutionary conserved histone variant that replaces conventional H2A in a subset of nucleosomes. The nucleosome is the basic unit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histones H2A, H2B, H3 and H4 (1).

 

 

Protein details:

 

Recombinant human histone H2A.X was produced in E. Coli, purified using FPLC and formulated in a storage buffer containing 20mM sodium phosphate pH 7.0, 1mM EDTA, 0.3 M NaCl, 0.5mM PMSF and 1mM DTT. Protein concentration was determined by spectrometry.

 

 

Quality control:

 

Each lot has been evaluated by ESI-TOF analysis and 12% SDS-PAGE (NuPAGE MOPS SDS, Invitrogen).

 

center    

SDS-PAGE gel of recombinant Histone H2A.X (Lane 2). Lane 1, protein molecular weight marker.

   

 

Application Notes:

 

For research use only.

 

 

Storage:

 

-80C

 

 

Guarantee:

 

Products guaranteed stable for 2 years from date of receipt when stored properly.

 

 

Purity:

 

>98% purity by SDS PAGE.

 

 

Protein sequences:

H2A.X (Theoretical Mass (Da):15,145)

MSGRGKTGGK ARAKAKSRSS RAGLQFPVGR VHRLLRKGHY AERVGAGAPV

YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGG

VTIAQGGVLP NIQAVLLPKK TSATVGPKAP SGGKKATQAS QEY

 

References:

1. Luger et al., (1997) Nature, 389(6648):251-60.

 

 

 

 


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