Human Native Nucleosome

Header

 

Catalog # : EPX-01-NCN

 

Source : Human

 

Quantity: 20 ug of Native Nucleosomes at 0.2ug/ul

 

 

 

 

Background:

 

The nucleosome is the basic subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone (H2A, H2B, H3 and H4).

 

 

Application Notes:

 

Suitable for in vitro chromatin assembly and enzyme activity assays.

 

 

Details:

 

Human native nucleosomes purified from HeLa cell nuclei, digested with micrococcal nuclease, and purified by glycerol gradient and ion exchange chromatography.

 

 

Presentation:

 

In buffer containing 1X Phosphate Buffered Saline (PBS), 0.5mM PMSF, and 50% glycerol.

 

 

 

 

Quality control:

 

Human native nucleosomes are evaluated by native gel shift assay.

 

 

Storage:

 

-80C

 

 

Guarantee:

 

For research use only. Products guaranteed stable for 1 year from date of receipt when stored properly.

 

 

Purity:

 

>98% purity by SDS PAGE.

 

 

 


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